منابع مشابه
Determination of Tocotrienols and Tocophenrols Using HPLC-ECD
Figure 1. Structures of the various tocopherol and tocotrienol vitamers. Introduction There are eight vitamers of vitamin E (alpha-, beta-, gamma-, and delta-tocopherols and the corresponding tocotrienols) (Figure 1), differing in their biological activity and distribution in foods. Most often vitamin E’s role is usually associated with its antioxidant activity. For example, α-tocopherol is a w...
متن کاملMeasurement of 8-OHdG in DNA by HPLC/ECD: the importance of DNA purity.
DNA has been considered to be pure when the ratio of absorbance at 260 nm over the absorbance at 280 nm (A260/A280) is 1.8 (8). This definition of purity, however, is based more upon a functional assessment (e.g., ability to cut the DNA with restriction enzymes) than upon more conventional analytical criteria. In a recent article, Glasel reexamined the significance of the A260/A280 (3). He note...
متن کاملBiological variability dominates and influences analytical variance in HPLC-ECD studies of the human plasma metabolome
BACKGROUND Biomarker-based assessments of biological samples are widespread in clinical, pre-clinical, and epidemiological investigations. We previously developed serum metabolomic profiles assessed by HPLC-separations coupled with coulometric array detection that can accurately identify ad libitum fed and caloric-restricted rats. These profiles are being adapted for human epidemiology studies,...
متن کاملBiomimetic synthesis and HPLC–ECD analysis of the isomers of dracocephins A and B
Starting from racemic naringenin ((±)-1), a mixture of dracocephin A stereoisomers 6-(2"-pyrrolidinone-5"-yl)naringenin (±)-2a-d and its regioisomer, dracocephin B 8-(2"-pyrrolidinone-5"-yl)naringenin (±)-3a-d originally isolated from Dracocephalum rupestre, have been synthesized in a one-pot reaction. The separation of 2a-d and 3a-d was achieved by preparative HPLC. The four stereoisomers of e...
متن کاملDirect Determination of ECD in ECD Kit: A Solid Sample Quantitation Method for Active Pharmaceutical Ingredient in Drug Product
Technetium-99m ethyl cysteinate dimer (Tc-99m-ECD) is an essential imaging agent used in evaluating the regional cerebral blood flow in patients with cerebrovascular diseases. Determination of active pharmaceutical ingredient, that is, L-Cysteine, N, N'-1,2-ethanediylbis-, diethyl ester, dihydrochloride (ECD) in ECD Kit is a relevant requirement for the pharmaceutical quality control in process...
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 1990
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.30.40